Test Bank For Microbiology Fundamentals A Clinical Approach 2nd Edition by Marjorie Kelly Cowan
Chapter 02
Tools of the Laboratory: The Methods for Studying Microorganisms
Multiple Choice Questions
1. The Five I’s of studying microorganisms include all of the following except
A. inoculation.
B. incubation.
C. infection.
D. isolation.
E. identification.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
2. The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is
A. isolation.
B. inoculation.
C. immunization.
D. infection.
E. contamination.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
3. A pure culture contains
A. only one species of microorganism.
B. only bacteria.
C. a variety of microbes from one source.
D. a variety of species from the same genus.
E. None of the choices is correct.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
4. The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the
A. colony.
B. inoculum.
C. streak.
D. loop.
E. None of the choices is correct.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
5. Which of the following is essential for development of discrete, isolated colonies?
A. broth medium
B. differential medium
C. selective medium
D. solid medium
E. assay medium
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
6. Which method often results in colonies developing down throughout the agar along with some colonies on the surface?
A. streak plate
B. spread plate
C. pour plate
D. All of the choices are correct.
E. None of the choices is correct.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
7. What type of isolation technique is most effective for the majority of applications?
A. pour plate
B. streak plate
C. spread plate
D. loop dilution
E. culture plate
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I’s mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
8. Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?
A. a pure culture
B. a mixed culture
C. a solid medium
D. a liquid medium
E. a contaminated medium
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
9. Agar is an important component of media because
A. bacteria require agar to grow.
B. agar inhibits mold growth.
C. agar provides a solid surface for bacterial growth.
D. agar prevents contamination.
E. All of the choices are correct.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
10. The three physical forms of laboratory media are
A. solid, liquid, and gas.
B. solid, semisolid, and liquid.
C. streak plate, pour plate, and broth.
D. aerobic, anaerobic, and micro aerobic.
E. None of the choices is correct.
ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom’s Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms
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